Thin-Layer Chromatography (TLC)

Description of Necessary Equipment

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This is a collection of the equipment you will be using (some of it shared):
  • TLC plate (silica gel) to left of pencil - handle only by edges!
  • Pencil (not pen) for marking the TLC plate.
  • Plastic ruler - metric scale.
  • Lined paper or index card for accurate spotting (held with blue tape in photo - a few set up for sharing, bring your own for convenience if you wish).
  • Developing jar (Check out one jar and one TLC plate for each solvent - three typically.)
  • Large filter paper - for lining of jar to "wick" solvent up, and thus saturate atmosphere in jar with solvent vapor.
  • Microcapillaries (You only need one - for your unknown; leave capillary dispenser in hood.)
  • Standard samples (vials standing in red tray - one set for entire lab; leave in hood)
©2001,2002 Daniel A. Straus

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